I have set-up the GC-FID again and things are running fine. The system works after I had a few problems with broken connectors. However, the baseline (BL) has stabilised at 16 pA after several days of equilibration. <\/p>\n
I have therefore cut the column at the detector end to reduce column bleeding that had occasionally occurred – no luck. The spikes that appeared every once in a while were mostly gone, but the BL stayed at 16 pA (identical with the BL of my colleague running a similar system next to me). In Alert, however, my BL was much better (4-5 pA). When cleaning the system I found out that my BL dropped to 13 pA, strange given the higher temperature and load on the column.<\/p>\n
But my hypothesis is that the (standard) compressed air is the culprit. The cleaning program uses a lower air-flow, thus introducing less contamination into the detector system. Additionally, I have used Ultra-zero air in Alert (much cleaner), which I have ordered now and that is due to arrive on Monday. <\/p>\n","protected":false},"excerpt":{"rendered":"
I have set-up the GC-FID again and things are running fine. The system works after I had a few problems with broken connectors. However, the baseline (BL) has stabilised at 16 pA after several days of equilibration. I have therefore cut the column at the detector end to reduce column bleeding that had occasionally occurred … Continue reading GC-FID baseline challenge<\/span><\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[4],"tags":[],"class_list":["post-119","post","type-post","status-publish","format-standard","hentry","category-research"],"_links":{"self":[{"href":"http:\/\/www.nomad.priv.at\/researchblog\/index.php?rest_route=\/wp\/v2\/posts\/119","targetHints":{"allow":["GET"]}}],"collection":[{"href":"http:\/\/www.nomad.priv.at\/researchblog\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/www.nomad.priv.at\/researchblog\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/www.nomad.priv.at\/researchblog\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"http:\/\/www.nomad.priv.at\/researchblog\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=119"}],"version-history":[{"count":0,"href":"http:\/\/www.nomad.priv.at\/researchblog\/index.php?rest_route=\/wp\/v2\/posts\/119\/revisions"}],"wp:attachment":[{"href":"http:\/\/www.nomad.priv.at\/researchblog\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=119"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/www.nomad.priv.at\/researchblog\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=119"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/www.nomad.priv.at\/researchblog\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=119"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}