Yesterday was another one with instrument time and things went well. I am still tweaking a couple of parameters (mainly injection conditions), but I am already collecting data (and I see quite a few species – some of them confirming earlier results). I am still struggling with two aspects of the measurements (that have to do with the splitless injection):<\/p>\n
(1) Column bleeding
\n(2) Decay of fiber signal after desorption<\/p>\n
I can reduce significantly (1) by choosing a short time until purging (1.5 min), but obviously my signals suffer. If I go by the typical desorption time of 5 min (resulting in purging after 5 min as well), I have a lot of column bleeding, which is not necessarily overlapping with analyte peaks, but messung up my chromatogram considerably.<\/p>\n
The same is true for (2) – after desorption I observe a large peak of (mainly) water desorbing from my fiber. By purging early, I can avoid that some of the water is loaded onto the column, resulting in a acceptable baseline earlier (and thus making this timeframe available for additional peaks), but of course my sensitivity suffers. <\/p>\n
Well, probably, it is going to be somewhere in the middle, although I lack a good selection criterion so far. But on the whole, it is going well.<\/p>\n","protected":false},"excerpt":{"rendered":"
Yesterday was another one with instrument time and things went well. I am still tweaking a couple of parameters (mainly injection conditions), but I am already collecting data (and I see quite a few species – some of them confirming earlier results). I am still struggling with two aspects of the measurements (that have to … Continue reading GC-MS measurements in full swing<\/span><\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[4],"tags":[],"class_list":["post-19","post","type-post","status-publish","format-standard","hentry","category-research"],"_links":{"self":[{"href":"http:\/\/www.nomad.priv.at\/researchblog\/index.php?rest_route=\/wp\/v2\/posts\/19","targetHints":{"allow":["GET"]}}],"collection":[{"href":"http:\/\/www.nomad.priv.at\/researchblog\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/www.nomad.priv.at\/researchblog\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/www.nomad.priv.at\/researchblog\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"http:\/\/www.nomad.priv.at\/researchblog\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=19"}],"version-history":[{"count":0,"href":"http:\/\/www.nomad.priv.at\/researchblog\/index.php?rest_route=\/wp\/v2\/posts\/19\/revisions"}],"wp:attachment":[{"href":"http:\/\/www.nomad.priv.at\/researchblog\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=19"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/www.nomad.priv.at\/researchblog\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=19"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/www.nomad.priv.at\/researchblog\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=19"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}