{"id":39,"date":"2005-08-31T15:40:34","date_gmt":"2005-08-31T20:40:34","guid":{"rendered":"http:\/\/www.nomad.priv.at\/researchblog\/?p=39"},"modified":"2005-08-31T15:40:34","modified_gmt":"2005-08-31T20:40:34","slug":"13c-containing-metabolite-monitoring-using-gc-ms","status":"publish","type":"post","link":"http:\/\/www.nomad.priv.at\/researchblog\/?p=39","title":{"rendered":"13C-containing Metabolite Monitoring Using GC-MS"},"content":{"rendered":"<p>Well, well &#8211; I have now analysed my GC-MS data from the first batch of <sup>13<\/sup>C malonic acid doped snow samples that I have incubated for one week now. No luck so far. Because of their heterogeneous and complex nature, the number of peaks was huge (160 in a 21 min run). I have tried to browse through the large peaks as well as the tiny humps in the baseline in order not to miss anything.<\/p>\n<p>What I had hoped for was a compound spectrum that could still be correctly identified by the NIST Mass Spectral Database or that I know from past runs with one of the masses shifted from n to n+1, due to the <sup>13<\/sup>C doping. However, this implies that the metabolite originates from a defined biochemical process and that it is an end-product of this pathway. This is pretty steep to begin with and probably I should dig more into the literature, although so far I have not found anything of relevance (especially for real life samples with unknown end-products).<\/p>\n<p>Should more than processes metabolise the <sup>13<\/sup>C, or should there be more than one end-product, then things get tricky, because the characteristical fragment that I am looking out for will be a lot less intense.<\/p>\n<p>Anyway &#8211; more work and more measurements ahead. Wish me luck and patience \ud83d\ude09<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Well, well &#8211; I have now analysed my GC-MS data from the first batch of 13C malonic acid doped snow samples that I have incubated for one week now. No luck so far. Because of their heterogeneous and complex nature, the number of peaks was huge (160 in a 21 min run). I have tried &hellip; <a href=\"http:\/\/www.nomad.priv.at\/researchblog\/?p=39\" class=\"more-link\">Continue reading <span class=\"screen-reader-text\">13C-containing Metabolite Monitoring Using GC-MS<\/span><\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[4],"tags":[],"class_list":["post-39","post","type-post","status-publish","format-standard","hentry","category-research"],"_links":{"self":[{"href":"http:\/\/www.nomad.priv.at\/researchblog\/index.php?rest_route=\/wp\/v2\/posts\/39","targetHints":{"allow":["GET"]}}],"collection":[{"href":"http:\/\/www.nomad.priv.at\/researchblog\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/www.nomad.priv.at\/researchblog\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/www.nomad.priv.at\/researchblog\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"http:\/\/www.nomad.priv.at\/researchblog\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=39"}],"version-history":[{"count":0,"href":"http:\/\/www.nomad.priv.at\/researchblog\/index.php?rest_route=\/wp\/v2\/posts\/39\/revisions"}],"wp:attachment":[{"href":"http:\/\/www.nomad.priv.at\/researchblog\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=39"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/www.nomad.priv.at\/researchblog\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=39"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/www.nomad.priv.at\/researchblog\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=39"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}