I have conducted some standard runs in the past weeks and set up 13 calibration curves for aromatic species. These include toluene, benzene, benzaldehyde and several others. I have also removed the water as described below and achieved some very good results.<\/p>\n
My sensitivity has increased by about 2 orders of magnitude – my smallest standard used to be 1.25 ug\/L, now it is 0.01 ug\/L and I think that I can still go lower for most of the species. The reason is the reduced amount of water that enters the system, giving me a better gain on the splitless injection, which loads all of my analytes on the column. I have not calculated an LOD yet, because I need more replicates for that, but it will improve drastically.<\/p>\n
My first calibration line covers a concentration range from 0.01 – 1000 ug\/L with decent linearity. However, with increasing concentration I have observed saturation of fiber, i.e. the loading capacity has been reached, leading to a plateau at high concentrations, suggesting a curvilinear calibration line.<\/p>\n
As a consequence I will set up different calibration lines for several concentration ranges and add more standards. I will also consider diluting highly concentrated samples, should this be necessary. From what I have seen so far, this should not be necessary, because observed concentrations were considerably lower than last year.<\/p>\n","protected":false},"excerpt":{"rendered":"
I have conducted some standard runs in the past weeks and set up 13 calibration curves for aromatic species. These include toluene, benzene, benzaldehyde and several others. I have also removed the water as described below and achieved some very good results. My sensitivity has increased by about 2 orders of magnitude – my smallest … Continue reading SPME-GC\/MS – increased sensitivity<\/span><\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[4],"tags":[],"class_list":["post-45","post","type-post","status-publish","format-standard","hentry","category-research"],"_links":{"self":[{"href":"http:\/\/www.nomad.priv.at\/researchblog\/index.php?rest_route=\/wp\/v2\/posts\/45","targetHints":{"allow":["GET"]}}],"collection":[{"href":"http:\/\/www.nomad.priv.at\/researchblog\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/www.nomad.priv.at\/researchblog\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/www.nomad.priv.at\/researchblog\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"http:\/\/www.nomad.priv.at\/researchblog\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=45"}],"version-history":[{"count":0,"href":"http:\/\/www.nomad.priv.at\/researchblog\/index.php?rest_route=\/wp\/v2\/posts\/45\/revisions"}],"wp:attachment":[{"href":"http:\/\/www.nomad.priv.at\/researchblog\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=45"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/www.nomad.priv.at\/researchblog\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=45"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/www.nomad.priv.at\/researchblog\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=45"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}